Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP769Y] to c-Myb (phospho S11)
Suitable for: WB, IHC-P, IP, Dot blot
Reacts with: Human

Product name

Anti-c-Myb (phospho S11) antibody [EP769Y]
See all c-Myb primary antibodies
Description

Rabbit monoclonal [EP769Y] to c-Myb (phospho S11)
Host species

Rabbit

Tested Applications & Species

Application	Species
Dot	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human c-Myb aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P10242
Positive control
- WB: Ramos, Molt-4, and HL-60 cell lysate. HeLa cell lysate untreated and treated with lambda phosphatase. IHC-P: Human cervical carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP769Y
Isotype

IgG
Research areas

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)

Lane 1 : Molt-4 (Human lymphoblastic leukemia cell line) cell lysate with c-Myb (phospho S11) peptide
Lane 2 : Molt-4 cell lysate with c-Myb (phospho S12) peptide
Lane 3 : Molt-4 cell lysate with c-Myb (phospho S11/12) peptide
Lane 4 : Molt-4 cell lysate with c-Myb unmodified peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 72 kDa
Observed band size: 77 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Immunohistochemical analysis of paraffin-embedded human vervical carcinoma tissue staining c-Myb with unpurified ab45150.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Dot blot analysis of c-Myb (phospho S11/12) peptide (Lane 1) and c-Myb unmodified peptide (Lane 2) labeling c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate with c-Myc (phospho S11) peptide
Lane 2 : HeLa cell lysate with c-Myc (phospho S12) peptide
Lane 3 : HeLa cell lysate with c-Myc (phospho S11/12) peptide
Lane 4 : HeLa cell lysate with c-Myc unmodified peptide

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 72 kDa
Observed band size: 75,77 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution (purified)

Lane 1 : Untreated Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : Ramos cell lysate, membrane treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 72 kDa
Observed band size: 75,77 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution (purified)

Lane 1 : Untreated HL-60 (Human promyelocytic leukemia cell line) cell lysate
Lane 2 : HL-60 cell lysate, membrane treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 72 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)

Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa cell lysate, the membrane treated with lambda phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 72 kDa
Observed band size: 75,77 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/10000 dilution (unpurified) + Ramos (Human Burkitt's lymphoma cell line) membrane lysate

Predicted band size: 72 kDa
Observed band size: 72,75 kDa why is the actual band size different from the predicted?
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com