Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
![Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412262-AntiZO1-tight-junction-protein-antibody-BLR092G-westernblot-A549-K562-HEK293T-MCF7-Jurkat-HeLa-LNCaP-and-RKO-cells.JPG "Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR092G] to ZO1 tight junction protein
- Suitable for: IHC-P, ICC, WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ZO1 tight junction protein antibody [BLR092G]
See all ZO1 tight junction protein primary antibodies -
Description
Rabbit monoclonal [BLR092G] to ZO1 tight junction protein -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human ZO1 tight junction protein aa 650-700. The exact sequence is proprietary. NP_003248.3
Database link: Q07157 -
Positive control
- WB: A549, K562, MCF7, HeLa, LNCaP, RKO; mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 whole cell lysate. IP: A549 whole cell lysate ICC: A549 cells. IHC-P: Human kidney, prostate carcinoma. IHC-P -IF: Human kidney.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 98% Borate buffered saline -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
BLR092G -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)All lanes : Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : HeLa whole cell lysate
Lane 7 : LNCaP whole cell lysate
Lane 8 : RKO whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 195 kDaChemiluminescence with an exposure time of 10 seconds.
Lower Panel: Rabbit antiCOPB2 antibody.
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![Immunocytochemistry - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412291-AntiZO1-tightjunctionprotein-antibody-BLR092G-immunocytochemistry-A549-cells.JPG)
Immunocytochemistry - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)A549 cells stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunocytochemical analysis. Substrate: DAB
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412286-AntiZO1-tightjunctionprotein-antibody-BLR092G-immunohistochemistry-prostate-carcinoma-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)Paraffin embedded human prostate carcinoma tissue stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used was HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412281-AntiZO1-tightjunctionprotein-antibody-BLR092G-immunohistochemistry-kidney-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)Paraffin embedded human kidney tissue stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used was HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412280-AntiZO1-tight-junction-protein-antibody-BLR092G-immunohistochemistryIF-kidney-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)Paraffin embedded human kidney tissue stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunohistochemical analysis. Secondary antibody was used HRP-conjugated goat antirabbit IgG, with counterstaining seen in blue (DAPI).
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![Immunoprecipitation - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412277-AntiZO1tightjunctionproteinantibodyBLR092GimmunoprecipitationA549cellshumn.jpg)
Immunoprecipitation - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells was prepared using NETN lysis buffer. ab276131 was used for IP at 12 µl per reaction (Lane 1). TJP1/ZO-1 was also immunoprecipitated by rabbit anti-TJP1/ZO-1 antibody. For blotting immunoprecipitated ab276131 was used at 1/1000 dilution. Contol IgG shown in Lane 2.
Chemiluminescence with an exposure time of 30 seconds
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![Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-412267-AntiZO1-tight-junction-protein-antibody-BLR092G-westernblot-mIMCD3-RenCa-NIH-3T3-TCMK1-and-C2C12-cells.JPG)
Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)All lanes : Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131) at 1/1000 dilution
Lane 1 : mIMCD-3 whole cell lysate
Lane 2 : RenCa whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : C2C12 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 195 kDaChemiluminescence with an exposure time of 3 minutes.
Lower Panel: Rabbit anti-COPB2 antibody.
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![Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)](https://www.abcam.com/ps/products/276/ab276131/Images/ab276131-8-benefits-of-recombinant-antibodies.png)
Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
