Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR092G] to ZO1 tight junction protein
  • Suitable for: IHC-P, ICC, WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ZO1 tight junction protein antibody [BLR092G]
    See all ZO1 tight junction protein primary antibodies

  • Description

    Rabbit monoclonal [BLR092G] to ZO1 tight junction protein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ZO1 tight junction protein aa 650-700. The exact sequence is proprietary. NP_003248.3
    Database link: Q07157

  • Positive control

    • WB: A549, K562, MCF7, HeLa, LNCaP, RKO; mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 whole cell lysate. IP: A549 whole cell lysate ICC: A549 cells. IHC-P: Human kidney, prostate carcinoma. IHC-P -IF: Human kidney.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 8.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 98% Borate buffered saline
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    BLR092G

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    All lanes : Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : HEK-293T whole cell lysate
    Lane 4 : MCF7 whole cell lysate
    Lane 5 : Jurkat whole cell lysate
    Lane 6 : HeLa whole cell lysate
    Lane 7 : LNCaP whole cell lysate
    Lane 8 : RKO whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 195 kDa



    Chemiluminescence with an exposure time of 10 seconds.

    Lower Panel: Rabbit antiCOPB2 antibody.

  • Immunocytochemistry - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Immunocytochemistry - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

    A549 cells stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunocytochemical analysis. Substrate: DAB

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

    Paraffin embedded human prostate carcinoma tissue stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used was HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

    Paraffin embedded human kidney tissue stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunohistochemical analysis. Secondary antibody used was HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

    Paraffin embedded human kidney tissue stained for ZO1 tight junction protein using ab276131 at 1/500 dilution in immunohistochemical analysis. Secondary antibody was used HRP-conjugated goat antirabbit IgG, with counterstaining seen in blue (DAPI).

  • Immunoprecipitation - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Immunoprecipitation - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

    Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells was prepared using NETN lysis buffer.  ab276131 was used for IP at 12 µl per reaction (Lane 1). TJP1/ZO-1 was also immunoprecipitated by rabbit anti-TJP1/ZO-1 antibody. For blotting immunoprecipitated ab276131 was used at 1/1000 dilution. Contol IgG shown in Lane 2.

    Chemiluminescence with an exposure time of 30 seconds

  • Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Western blot - Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    All lanes : Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131) at 1/1000 dilution

    Lane 1 : mIMCD-3 whole cell lysate
    Lane 2 : RenCa whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : C2C12 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 195 kDa



    Chemiluminescence with an exposure time of 3 minutes.

    Lower Panel: Rabbit anti-COPB2 antibody.

  • Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)
    Anti-ZO1 tight junction protein antibody [BLR092G] (ab276131)

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