Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19945-296] to ZO1 tight junction protein - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free
    See all ZO1 tight junction protein primary antibodies

  • Description

    Rabbit monoclonal [EPR19945-296] to ZO1 tight junction protein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IHC-Fr,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251568 is the carrier-free version of ab221547. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251568 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19945-296

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Immunocytochemistry - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)

    This data was developed using ab221547, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling ZO1 tight junction protein with ab221547 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunocytochemistry - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Immunocytochemistry - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)

    This data was developed using ab221547, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells labeling ZO1 tight junction protein with ab221547 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HEK-293 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Flow Cytometry - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Flow Cytometry - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    This data was developed using ab221547, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling ZO1 tight junction protein with ab221547 at 1/60 dilution (red) compared with an Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    This data was developed using ab221547, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ZO1 tight junction protein with ab221547 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in vascular endothelial cells and glomerular of human kidney (PMID: 18618131) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    This data was developed using ab221547, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ZO1 tight junction protein with ab221547 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining in endothelial cells of rat spleen (PMID: 17585317) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    This data was developed using ab221547, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ZO1 tight junction protein with ab221547 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in glomerular of mouse kidney (PMID:17585317; PMID: 18618131) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    This data was developed using ab221547, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ZO1 tight junction protein with ab221547 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in breast cancer (PMID: 9846967) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)
    Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free (ab251568)

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