Anti-c-Myb antibody [EPR718(2)] (ab109127)
![Anti-c-Myb antibody [EPR718(2)] (ab109127)](https://www.abcam.com/ps/products/109/ab109127/Images/ab109127-296659-antic-myb-antibody-epr7182-western-blot-human-hap1.jpg "Anti-c-Myb antibody [EPR718(2)] (ab109127)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR718(2)] to c-Myb
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-c-Myb antibody [EPR718(2)]
See all c-Myb primary antibodies -
Description
Rabbit monoclonal [EPR718(2)] to c-Myb -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat and MOLT4 cell lysates; Human colon tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR718(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. Ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)](https://www.abcam.com/ps/products/109/ab109127/Images/ab109127-121724-anti-c-myb-antibody-epr7182-western-blot.jpg)
Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)All lanes : Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : MOLT4 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa
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![Anti-c-Myb antibody [EPR718(2)] (ab109127)](https://www.abcam.com/ps/products/109/ab109127/Images/ab109127-4-benefits-of-recombinant-antibodies.png)
Anti-c-Myb antibody [EPR718(2)] (ab109127)
