Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CNOT6 antibody [EPR22022] (ab221151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22022] to CNOT6
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CNOT6 antibody [EPR22022]
    See all CNOT6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22022] to CNOT6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat and MCF7 whole cell lysates. IP: Jurkat and HeLa whole cell lysates. Human testis, ovaries and heart tissue lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22022

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1 : Human testis lysate with 5% NFDM/TBST
    Lane 2 : Human ovary lysate with 5% NFDM/TBST
    Lane 3 : Human heart lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    The expression profile is consistent with what has been described in the literature (PMID: 21741365).

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1 : His-tagged human CNOT6 recombinant protein(aa1-210) with 5% NFDM/TBST
    Lane 2 : His-tagged human CNOT6L recombinant protein(aa1-210) with 5% NFDM/TBST

    Lysates/proteins at 0.002 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa


    Exposure time: 3 seconds
  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa whole cell lysate with CNOT6 Immunogen peptide

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The reactivity of the antibody with the target protein in the HeLa lysate was blocked by the inclusion of the peptide antigen.

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)
    All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : Human ovary cancer lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 63 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)
    Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

    CNOT6 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab221151 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)
    Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

    CNOT6 was immunoprecipitated from 0.35 mg of Jurkat (human  T cell leukemia cell line from peripheral blood) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate 10 µg (Input). 

    Lane 2: ab221151 IP in Jurkat whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-CNOT6 antibody [EPR22022] (ab221151)
    Anti-CNOT6 antibody [EPR22022] (ab221151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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