Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Friday March 12, 2021

Anti-CNOT6 antibody [EPR22022] (ab221151)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22022] to CNOT6
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-CNOT6 antibody [EPR22022]
See all CNOT6 primary antibodies
Description

Rabbit monoclonal [EPR22022] to CNOT6
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Jurkat and MCF7 whole cell lysates. IP: Jurkat and HeLa whole cell lysates. Human testis, ovaries and heart tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22022
Isotype

IgG
Research areas

Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

Lane 1 : Human testis lysate with 5% NFDM/TBST
Lane 2 : Human ovary lysate with 5% NFDM/TBST
Lane 3 : Human heart lysate with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 63 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

Exposure time: 37 seconds

The expression profile is consistent with what has been described in the literature (PMID: 21741365).
Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

Lane 1 : His-tagged human CNOT6 recombinant protein(aa1-210) with 5% NFDM/TBST
Lane 2 : His-tagged human CNOT6L recombinant protein(aa1-210) with 5% NFDM/TBST

Lysates/proteins at 0.002 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 63 kDa

Exposure time: 3 seconds
Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa whole cell lysate with CNOT6 Immunogen peptide

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 63 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The reactivity of the antibody with the target protein in the HeLa lysate was blocked by the inclusion of the peptide antigen.
Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

All lanes : Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Human ovary cancer lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 63 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

CNOT6 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).

Lane 2: ab221151 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

CNOT6 was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1: Jurkat whole cell lysate 10 µg (Input).

Lane 2: ab221151 IP in Jurkat whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221151 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-CNOT6 antibody [EPR22022] (ab221151)

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