Crystal violet Assay Kit (Cell viability) (ab232855)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells
Overview
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Product name
Crystal violet Assay Kit (Cell viability) -
Detection method
Colorimetric -
Sample type
Adherent cells -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Crystal Violet Assay Kit ab232855 is used for cytoxicity and cell viability studies with adherent cell cultures.
The Crystal Violet assay is based on staining cells that are attached to cell culture plates. It relies on the detachment of adherent cells from cell culture plates during cell death. During the assay, dead detached cells are washed away. The remaining attached live cells are stained with Crystal violet. After a wash step, the Crystal violet dye is solubilized and measured by absorbance at 595 nm.
The amount of Crystal Violet staining in the assay is directly proportional to the cell biomass that is attached to the plate. The cell biomass can be used to infer levels of cell viability / cytotoxicity. The Crystal Violet assay assumes that all cells that are attached to the plate are "alive" and that all cells that detach are "dead".
Crystal violet is a triarylmethane dye that binds to ribose type molecules such as DNA in nuclei.
Crystal Violet assay protocol summary:
- remove culture medium
- wash cells
- add Crystal Violet staining solution
- incubate for 20 mins
- wash cells and air dry plate
- add solubilization solution
- analyze using a microplate reader at 595 nm absorbance -
Notes
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1000 tests Crystal violet Staining Solution 1 x 40ml 10X Washing Solution 1 x 115ml Solubilization Solution 1 x 100ml 20 mM Doxorubicin 1 x 100µl
Images
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Dose-response curve of MCF7 (human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).
Dose-response curve of MCF7 (Human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.
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Dose-response curve of HepG2 (human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability) (ab232855).Dose-response curve of HepG2 (Human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet staining assay. Assays were performed according to the kit protocol in triplicate.