DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
![DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)](https://www.abcam.com/ps/products/117/ab117504/Images/ab117504-276742-anti-6x-his-tag-antibody-ad1110-dylight-650-immunofluorescence.jpg "DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)")
Key features and details
- DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Isotype: IgG1
Overview
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Product name
DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
DyLight® 650 Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm -
Specificity
ab117504 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.
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Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresonding to 6X His tag®.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504) This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.
