HRP Anti-6X His tag® antibody [GT359] (ab184607)
![HRP Anti-6X His tag® antibody [GT359] (ab184607)](https://www.abcam.com/ps/products/184/ab184607/Images/ab184607-215388-anti-6x-his-tag-antibody-gt359-hrp-western-blot.jpg "HRP Anti-6X His tag® antibody [GT359] (ab184607)")
Key features and details
- HRP Mouse monoclonal [GT359] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG3
Overview
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Product name
HRP Anti-6X His tag® antibody [GT359]
See all 6X His tag® primary antibodies -
Description
HRP Mouse monoclonal [GT359] to 6X His tag® -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Positive control
- Cell lysate from E. coli expressing His-tagged proteins.
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General notes
This product was changed from ascites to tissue culture supernatant on 28th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.025% Proclin 300
Constituents: 1% BSA, 0.87% Sodium chloride, 0.65% Tris -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
GT359 -
Isotype
IgG3 -
Research areas
Images
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Western blot - HRP Anti-6X His tag® antibody [GT359] (ab184607)All lanes : HRP Anti-6X His tag® antibody [GT359] (ab184607) at 1/5000 dilution
Lane 1 : non-induced cell lysate from E. coli expressing a 6x His-tagged protein
Lane 2 : IPTG-induced cell lysate from E. coli expressing a 6x His-tagged protein
Lysates/proteins at 5 µl per lane.
Developed using the ECL technique.
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![Western blot - HRP Anti-6X His tag® antibody [GT359] (ab184607)](https://www.abcam.com/ps/products/184/ab184607/Images/ab184607-215387-anti-6x-his-tag-antibody-gt359-hrp-western-blot.jpg)
Western blot - HRP Anti-6X His tag® antibody [GT359] (ab184607)All lanes : HRP Anti-6X His tag® antibody [GT359] (ab184607) at 1/10000 dilution
Lanes 1-2 : IPTG-induced cell lysate from E. coli expressing a GST-tagged protein
Lanes 3-6 : IPTG-induced cell lysate from E. coli expressing a 6x His-tagged protein
Developed using the ECL technique.
