Anti-SEC23A antibody [EPR13270(B)] (ab179811)
![Anti-SEC23A antibody [EPR13270(B)] (ab179811)](https://www.abcam.com/ps/products/179/ab179811/Images/ab179811-261772-anti-sec23-antibody-epr13270b-western-blot.jpg "Anti-SEC23A antibody [EPR13270(B)] (ab179811)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13270(B)] to SEC23A
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SEC23A antibody [EPR13270(B)]
See all SEC23A primary antibodies -
Description
Rabbit monoclonal [EPR13270(B)] to SEC23A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human SEC23 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15436 -
Positive control
- HeLa, HepG2 and 293T cell lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13270(B) -
Isotype
IgG
Images
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Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SEC23A knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab179811 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.ab179811 was shown to specifically react with SEC23A when SEC23A knockout samples were used. Wild-type and SEC23A knockout samples were subjected to SDS-PAGE. ab179811 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811)](https://www.abcam.com/ps/products/179/ab179811/Images/ab179811-208076-anti-sec23-antibody-epr13270b-western-blot.jpg)
Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811)All lanes : Anti-SEC23A antibody [EPR13270(B)] (ab179811) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 86 kDa
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![Flow Cytometry - Anti-SEC23A antibody [EPR13270(B)] (ab179811)](https://www.abcam.com/ps/products/179/ab179811/Images/ab179811-208077-anti-sec23-antibody-epr13270b-flow-cytometry.jpg)
Flow Cytometry - Anti-SEC23A antibody [EPR13270(B)] (ab179811)Flow Cytometrical analysis of permeabilized HeLa cells labeling SEC23A with ab179811 at 1/10 (red) or a rabbit IgG (negative) (green).
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![Anti-SEC23A antibody [EPR13270(B)] (ab179811)](https://www.abcam.com/ps/products/179/ab179811/Images/ab179811-4-benefits-of-recombinant-antibodies.png)
Anti-SEC23A antibody [EPR13270(B)] (ab179811)
