Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SEC23A antibody [EPR13270(B)] (ab179811)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13270(B)] to SEC23A
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-SEC23A antibody [EPR13270(B)]
See all SEC23A primary antibodies
Description

Rabbit monoclonal [EPR13270(B)] to SEC23A
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human SEC23 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15436
Positive control
- HeLa, HepG2 and 293T cell lysates; HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13270(B)
Isotype

IgG

Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SEC23A knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab179811 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab179811 was shown to specifically react with SEC23A when SEC23A knockout samples were used. Wild-type and SEC23A knockout samples were subjected to SDS-PAGE. ab179811 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-SEC23A antibody [EPR13270(B)] (ab179811)

All lanes : Anti-SEC23A antibody [EPR13270(B)] (ab179811) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 86 kDa
Flow Cytometry - Anti-SEC23A antibody [EPR13270(B)] (ab179811)

Flow Cytometrical analysis of permeabilized HeLa cells labeling SEC23A with ab179811 at 1/10 (red) or a rabbit IgG (negative) (green).
Anti-SEC23A antibody [EPR13270(B)] (ab179811)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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