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Anti-C5a antibody [EPR19699-24] (ab202039)

Price and availability

314 937 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-C5a antibody [EPR19699-24] (ab202039)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19699-24] to C5a
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-C5a antibody [EPR19699-24]
    See all C5a primary antibodies
  • Description

    Rabbit monoclonal [EPR19699-24] to C5a
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human serum. IP: Human serum.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19699-24
  • Isotype

    IgG

Images

  • Western blot - Anti-C5a antibody [EPR19699-24] (ab202039)
    Western blot - Anti-C5a antibody [EPR19699-24] (ab202039)
    Anti-C5a antibody [EPR19699-24] (ab202039) at 1/1000 dilution + Human serum at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Exposure time: 3 minutes


    C5 is cleaved into C5α and C5β and this antibody recognizes the C5α. The molecular weight observed is consistent with what has been described in the literature (PMID: 23401592)

  • Immunoprecipitation - Anti-C5a antibody [EPR19699-24] (ab202039)
    Immunoprecipitation - Anti-C5a antibody [EPR19699-24] (ab202039)

    C5a was immunoprecipitated from 0.35 mg human serum 10μg with ab202039 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab202039 at 1/2000 dilution (0.26 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/10,000 dilution.

    Lane 1: Human serum 10μg.

    Lane 2: ab202039 IP in human serum.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202039 in human serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This antibody recognizes C5a.

  • Anti-C5a antibody [EPR19699-24] (ab202039)
    Anti-C5a antibody [EPR19699-24] (ab202039)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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