Antibodies, Proteins, Kits and Reagents for Life Science

Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3335(2)] to LIS1 - BSA and Azide free
Suitable for: Flow Cyt (Intra), WB
Reacts with: Human

Product name

Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free
See all LIS1 primary antibodies
Description

Rabbit monoclonal [EPR3335(2)] to LIS1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab247915 is the carrier-free version of ab109630.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3335(2)
Isotype

IgG
Research areas

Flow Cytometry - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

This data was developed using ab109630, the same antibody clone in a different buffer formulation.Overlay histogram showing SHSY-5Y cells stained with ab109630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109630, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

All lanes : Anti-LIS1 antibody [EPR3335(2)] (ab109630) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 47 kDa

This data was developed using ab109630, the same antibody clone in a different buffer formulation.
Flow Cytometry - Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

This data was developed using ab109630, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells using ab109630 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Anti-LIS1 antibody [EPR3335(2)] - BSA and Azide free (ab247915)

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