Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17848-87] to CYFIP2 + CYFIP1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87]
    See all CYFIP2 + CYFIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17848-87] to CYFIP2 + CYFIP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human CYFIP1 Recombinant protein fragment; Jurkat, HeLa, SW480 and Raw264.7 and Molt4 whole cell lysates; Human fetal kidney and fetal brain cell lysates. IHC-P: Human tonsil tissue. ICC/IF: Jurkat and SW480 cells. Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17848-87

  • Isotype

    IgG

Images

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/50000 dilution + Human CYFIP1 Recombinant protein fragment at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 148 kDa


    Exposure time: 10 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    All lanes : Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
    Lane 2 : Molt4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
    Lane 3 : Human fetal kidney lysate at 10 µg
    Lane 4 : Human fetal brain lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 148 kDa


    Exposure time: 1 second


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    All lanes : Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 148 kDa


    Exposure time: 15 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/1000 dilution + Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 146 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CYFIP2 + CYFIP1 with ab204129 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on human tonsil tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Immunofluorescent analysis of 100% Methanol, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on JurKat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab204129 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Immunocytochemistry/ Immunofluorescence - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Immunofluorescent analysis of 100% Methanol, 0.1% Triton X-100 permeabilized SW480 (Human colon adenocarcinoma cell line) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab204129 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Flow Cytometry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat  (Human T cell leukemia cells from peripheral blood) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)
    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129)

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