Anti-DDR2 antibody [EPR18826-119] (ab280354)
![Anti-DDR2 antibody [EPR18826-119] (ab280354)](https://www.abcam.com/ps/products/280/ab280354/Images/ab280354-456142-anti-ddr2-antibody-epr18826119-western-blot-epithelial.jpg "Anti-DDR2 antibody [EPR18826-119] (ab280354)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18826-119] to DDR2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DDR2 antibody [EPR18826-119]
See all DDR2 primary antibodies -
Description
Rabbit monoclonal [EPR18826-119] to DDR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-1080, HUVEC, A-204, A375, Human stomach, ovary, lung, Mouse ovary, lung, C2C12, C6, Rat ovary and His-tagged human DDR2 recombinant protein (aa22-399).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18826-119 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : A-204 (rat muscle rhabdomyosarcoma) whole cell lysate
Lane 4 : A375 (human malignant melanoma epithelial cell) whole cell lysate
Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Negative control: MDA-MB-231 (PMID: 28147276, 29616590)
Exposure time: 158 seconds.
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![Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)](https://www.abcam.com/ps/products/280/ab280354/Images/ab280354-456143-anti-ddr2-antibody-epr18826119-western-blot-lysate.jpg)
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : Human stomach tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Exposure time: 3 minutes.
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![Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)](https://www.abcam.com/ps/products/280/ab280354/Images/ab280354-456144-anti-ddr2-antibody-epr18826119-western-blot-lysate1.jpg)
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : Mouse ovary tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Exposure time: 3 minutes.
-
![Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)](https://www.abcam.com/ps/products/280/ab280354/Images/ab280354-456145-anti-ddr2-antibody-epr18826119-western-blot-tumor.jpg)
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : Rat ovary tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).
Exposure time: 3 minutes.
-
![Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)](https://www.abcam.com/ps/products/280/ab280354/Images/ab280354-456146-anti-ddr2-antibody-epr18826119-western-blot-recombinant.jpg)
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution
Lane 1 : His-tagged human DDR2 recombinant protein (aa22-399)
Lane 2 : His-tagged human DDR1 recombinant protein (aa21-416)
Lysates/proteins at 0.03 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody can not recognize human DDR1.
Both recombinant proteins are made in house and extracted from HEK-293 expression systems.
Exposure time: 8 seconds
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![Anti-DDR2 antibody [EPR18826-119] (ab280354)](https://www.abcam.com/ps/products/280/ab280354/Images/ab280354-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-DDR2 antibody [EPR18826-119] (ab280354)