Antibodies, Proteins, Kits and Reagents for Life Science

Anti-DDR2 antibody [EPR18826-119] (ab280354)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18826-119] to DDR2
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-DDR2 antibody [EPR18826-119]
See all DDR2 primary antibodies
Description

Rabbit monoclonal [EPR18826-119] to DDR2
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HT-1080, HUVEC, A-204, A375, Human stomach, ovary, lung, Mouse ovary, lung, C2C12, C6, Rat ovary and His-tagged human DDR2 recombinant protein (aa22-399).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18826-119
Isotype

IgG
Research areas
- Neuroscience
- Development

Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)

All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

Lane 1 : HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : A-204 (rat muscle rhabdomyosarcoma) whole cell lysate
Lane 4 : A375 (human malignant melanoma epithelial cell) whole cell lysate
Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

Negative control: MDA-MB-231 (PMID: 28147276, 29616590)

Exposure time: 158 seconds.
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)

All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

Lane 1 : Human stomach tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human lung tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

Exposure time: 3 minutes.
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)

All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

Lane 1 : Mouse ovary tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

Exposure time: 3 minutes.
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)

All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : Rat ovary tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 97 kDa
Observed band size: 120-130,70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression pattern is consistent with what has been described in the literature (PMID: 15111304, 29616590).

Exposure time: 3 minutes.
Western blot - Anti-DDR2 antibody [EPR18826-119] (ab280354)

All lanes : Anti-DDR2 antibody [EPR18826-119] (ab280354) at 1/1000 dilution

Lane 1 : His-tagged human DDR2 recombinant protein (aa22-399)
Lane 2 : His-tagged human DDR1 recombinant protein (aa21-416)

Lysates/proteins at 0.03 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 97 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody can not recognize human DDR1.

Both recombinant proteins are made in house and extracted from HEK-293 expression systems.

Exposure time: 8 seconds
Anti-DDR2 antibody [EPR18826-119] (ab280354)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDR2 antibody [EPR18826-119] (ab280354)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images