Recombinant rat PDE7A/HCP1 protein (ab198419)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant rat PDE7A/HCP1 protein
See all PDE7A/HCP1 proteins and peptides -
Biological activity
Specific Activity: ≥354 pmol/min/µg.
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198419 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
30 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMVKLRR FLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLANSVTPWDILLSL IAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSVLENHHWRSAVGLLRES GLFSHLPLESRHEMEAQIGALILATDISRQNEYLSLFRSHLDKGDLHLDD GRHRHLVLQMALKCADICNPCRNWELSKQWSEKVTEEFFHQGDIEKKYHL GVSPLCDRQTESIANIQIGFMTYLVEPLFTEWARFSDTRLSQTMLGHVGL NKASWKGLQRQQPSSEDASAAFELNSQLLTQENRLS -
Predicted molecular weight
65 kDa including tags -
Amino acids
147 to 482 -
Tags
GST tag N-Terminus
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Preparation and Storage
- 5''-cyclic phosphodiesterase 7A
- HCP 1
- HCP1
Images
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Functional Studies - Recombinant rat PDE7A/HCP1 protein (ab198419)
Activity assay using ab198419.
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SDS-PAGE - Recombinant rat PDE7A/HCP1 protein (ab198419)10% SDS-PAGE analysis of 5µg ab198419 with Coomassie staining.
