Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [YEYE311] to CSF-1-R - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free
    See all CSF-1-R primary antibodies

  • Description

    Rat monoclonal [YEYE311] to CSF-1-R - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 and J774A.1 whole cell lysate. IHC-P: Mouse liver and lymph node tissue. ICC/IF: RAW 264.7 cells.

  • General notes

    ab271304 is the carrier-free version of ab271294. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab271304 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)
    Western blot - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)
    All lanes : Anti-CSF-1-R antibody [YEYE311] (ab271294) at 1.208 µg/ml

    Lane 1 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 108 kDa


    Exposure time: 3 minutes


    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:14673177).
    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CSF1R with ab271294 at 1/500 dilution (2.416µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the Kupffer cells in the mouse liver. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)

    Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue labeling CSF1R with ab271294 at 1/500 (2.416µg/ml) dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on the macrophages in mouse lymph node. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

  • Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)
    Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling CSF1R with ab271294 at 1/50 dilution (24.16µg/ml), followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous and weak cytoplasmic staining in RAW 264.7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab271294 at a 1/50 dilution (24.16µg/ml) followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271294).

  • Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)
    Anti-CSF-1-R antibody [YEYE311] - BSA and Azide free (ab271304)

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