Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24003-118] to GABA A Receptor alpha 5/GABRA5 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free
    See all GABA A Receptor alpha 5/GABRA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR24003-118] to GABA A Receptor alpha 5/GABRA5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and hippocampus tissue lysates; Rat hippocampus tissue lysate. IHC-P: Mouse hippocampus tissue; Rat hippocampus tissue. IP: Mouse brain tissue lysate; Rat brain tissue lysate.

  • General notes

    ab281564 is the carrier-free version of ab259880. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24003-118

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

    This data was developed using ab259880, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labelling GABA A Receptor alpha 5/GABRA5 with ab259880 at 1/50 (11.4 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse hippocampus (PMID:27364953). The section was incubated with ab259880 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX. instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

    This data was developed using ab259880, the same antibody clone in a different buffer formulation.

    GABA A Receptor alpha 5/GABRA5 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab259880 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259880 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab259880 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259880 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds

    This blot was developed using a higher sensitivity ECL substrate.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

    This data was developed using ab259880, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labelling GABA A Receptor alpha 5/GABRA5 with ab259880 at 1/50 (11.4 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat hippocampus. The section was incubated with ab259880 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    All lanes : Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 52 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using 259880, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

     

  • Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Western blot - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] (ab259880) at 1/1000 dilution + Rat hippocampus tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 52 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using 259880, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

     

  • Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Immunoprecipitation - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

    This data was developed using ab259880, the same antibody clone in a different buffer formulation.

    GABA A Receptor alpha 5/GABRA5 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab259880 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259880 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab259880 IP in rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259880 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

    This data was developed using ab259880, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling GABA A Receptor alpha 5/GABRA5 with ab259880 at 1/50 (11.4 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on mouse spleen.The section was incubated with ab259880 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND  RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)
    Anti-GABA A Receptor alpha 5/GABRA5 antibody [EPR24003-118] - BSA and Azide free (ab281564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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