Mouse Angiopoietin 2 Matched Antibody Pair Kit (ab219520)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse Angiopoietin 2 Matched Antibody Pair Kit
See all Angiopoietin 2/ANG2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
4.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Angiopoietin 2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Angiopoietin 2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab209883. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Angiopoetin 2 Capture Antibody 2 x 50µg 1 x 50µg Mouse Angiopoetin 2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Angiopoetin 2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGPT 2
- Agpt2
- ANG 2
see all -
Database links
- Entrez Gene: 11601 Mouse
- SwissProt: O35608 Mouse
- Unigene: 439874 Mouse
Images
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Mouse Angiopoietin 2 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Angiopoietin 2 Matched Antibody Pair Kit (ab219520)To learn more about the advantages of recombinant antibodies see here.
