Mouse Angiopoietin 2 ELISA Kit (ab209883)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.3 pg/ml
- Range: 68.75 pg/ml - 4400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Angiopoietin 2 ELISA Kit
See all Angiopoietin 2/ANG2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse plasma 3 8.3% Inter-assay Sample n Mean SD CV% Mouse plasma 5 10.3% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17.3 pg/ml -
Range
68.75 pg/ml - 4400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.5 67.4% - 120.9% Cell culture media 99.7 94.3% - 103.3% Hep Plasma 112.4 81.4% - 143.2% EDTA Plasma 104.9 91.8% - 122.8% Cit plasma 94.7 87.1% - 103.3% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Angiopoietin 2 ELISA Kit (ab209883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin 2 with 17.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Angiopoietin 2 is a secreted glycosylated protein functioning in angiogenesis, inflammation and vascular development. Angiopoietin 2 binds to receptor tyrosine kinase TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signalling. Angiopoietin 2 can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, Angiopoietin 2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, Angiopoietin 2 may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Angiopoietin 2 Capture Antibody 1 x 600µl 10X Mouse Angiopoietin 2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Angiopoietin 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGPT 2
- Agpt2
- ANG 2
see all -
Database links
- Entrez Gene: 11601 Mouse
- SwissProt: O35608 Mouse
- Unigene: 439874 Mouse
Images
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Other - Mouse Angiopoietin 2 ELISA Kit (ab209883)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Angiopoietin 2 standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Angiopoietin 2 in mouse and rat serum and mouse plasma samplesThe concentrations of Angiopoietin 2 were measured in duplicates, interpolated from the Angiopoietin 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 6%, plasma (EDTA) 7.5%, plasma (heparin) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin 2 concentration was determined to be 19,017 pg/mL in mouse serum, 2,802 pg/mL in rat serum, 31,192 pg/mL in mouse plasma (citrate), 26,451 pg/mL in mouse plasma (EDTA) and 33,857 pg/mL in mouse plasma (heparin).
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Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (ab209883)To learn more about the advantages of recombinant antibodies see here.
