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Mouse Angiopoietin 2 ELISA Kit (ab209883)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 17.3 pg/ml
  • Range: 68.75 pg/ml - 4400 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Angiopoietin 2 ELISA Kit
    See all Angiopoietin 2/ANG2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse plasma 3 8.3%
    Inter-assay
    Sample n Mean SD CV%
    Mouse plasma 5 10.3%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    17.3 pg/ml

  • Range

    68.75 pg/ml - 4400 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94.5 67.4% - 120.9%
    Cell culture media 99.7 94.3% - 103.3%
    Hep Plasma 112.4 81.4% - 143.2%
    EDTA Plasma 104.9 91.8% - 122.8%
    Cit plasma 94.7 87.1% - 103.3%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse Angiopoietin 2 ELISA Kit (ab209883) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin 2 with 17.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Angiopoietin 2 is a secreted glycosylated protein functioning in angiogenesis, inflammation and vascular development. Angiopoietin 2 binds to receptor tyrosine kinase TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signalling. Angiopoietin 2 can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, Angiopoietin 2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, Angiopoietin 2 may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse Angiopoietin 2 ELISA Kit (ab209883)
    Other - Mouse Angiopoietin 2 ELISA Kit (ab209883)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse Angiopoietin 2 standard curve
    Example of mouse Angiopoietin 2 standard curve

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Angiopoietin 2 in mouse and rat serum and mouse plasma samples
    Interpolated concentrations of native Angiopoietin 2 in mouse and rat serum and mouse plasma samples

    The concentrations of Angiopoietin 2 were measured in duplicates, interpolated from the Angiopoietin 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 6%, plasma (EDTA) 7.5%, plasma (heparin) 7.5%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean Angiopoietin 2 concentration was determined to be 19,017 pg/mL in mouse serum, 2,802 pg/mL in rat serum, 31,192 pg/mL in mouse plasma (citrate), 26,451 pg/mL in mouse plasma (EDTA) and 33,857 pg/mL in mouse plasma (heparin).

  • Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (ab209883)
    Sandwich ELISA - Mouse Angiopoietin 2 ELISA Kit (ab209883)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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