Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRO antibody [EPR12158] (ab181048)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12158] to MRO
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MRO antibody [EPR12158]
    See all MRO primary antibodies

  • Description

    Rabbit monoclonal [EPR12158] to MRO

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human ovary cancer, fetal heart and fetal brain lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12158

  • Isotype

    IgG

Images

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    All lanes : Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution (purified)

    Lane 1 : mouse spleen lysate
    Lane 2 : rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution (purified) + human fetal heart lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    Anti-MRO antibody [EPR12158] (ab181048) at 1/5000 dilution (purified) + human fetal brain lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)
    Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

    ab181048 (purified) at 1/20 immunoprecipitating MRO in 10 μg human fetal heart whole cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    Western blot - Anti-MRO antibody [EPR12158] (ab181048)
    All lanes : Anti-MRO antibody [EPR12158] (ab181048) at 1/1000 dilution (unpurified)

    Lane 1 : Human ovary cancer lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 29 kDa

  • Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)
    Immunoprecipitation - Anti-MRO antibody [EPR12158] (ab181048)

    Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate (lane 1) or a negative control (lane 2) immunoprecipitated using unpurified ab181048 at 1/80 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-MRO antibody [EPR12158] (ab181048)
    Anti-MRO antibody [EPR12158] (ab181048)

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