Anti-ULK1 antibody (ab240916)
Key features and details
- Rabbit polyclonal to ULK1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ULK1 antibody
See all ULK1 primary antibodies -
Description
Rabbit polyclonal to ULK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human ULK1 aa 550-650 (internal sequence).
Database link: O75385 -
Positive control
- ICC/IF: SK-N-BE cells. WB: HeLa and human 293Trap cell lysate; Rat brain cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ULK1 antibody (ab240916)
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ULK1 (green) using ab240916 at 1/100 dilution in ICC/IF, followed by a goat anti-rabbit ATTO 488 conjugated secondary antibody at 1/200 dilution. Both primary and secondary antibodies were incubated for 1 hour at RT. Counterstained with Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 1 hour at RT. The nuclear counterstain in DAPI (blue) at 1/5000 for 5 minutes at RT.
(A) DAPI (blue) nuclear stain
(B) Phalloidin Texas Red F-Actin stain (red)
(C) ab240916
(D) Composite
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Western blot - Anti-ULK1 antibody (ab240916)All lanes : Anti-ULK1 antibody (ab240916) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Human 293Trap cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-linked goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 6 minutesBlocked with 5% skim milk in 1XTBST.
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Western blot - Anti-ULK1 antibody (ab240916)Anti-ULK1 antibody (ab240916) at 1/1000 dilution + Rat kidney lysate at 15 µg
Secondary
HRP-linked goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 6 minutes
