Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Friday March 05, 2021

Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6156] to ULK1 (phospho S556)
Suitable for: WB, Dot blot
Reacts with: Human

Product name

Anti-ULK1 (phospho S556) antibody [EPR6156]
See all ULK1 primary antibodies
Description

Rabbit monoclonal [EPR6156] to ULK1 (phospho S556)
Host species

Rabbit
Specificity

ab133747 only detects ULK1 phosphorylated at Serine 556.
Tested Applications & Species

Application Species
Dot
Human

WB
Human

See all applications and species data
Immunogen

Phospho-synthetic peptide from a region surrounding Serine 556 of Human ULK1
Positive control
- WB: ULK1 transfected HEK-293T cell lysate. HEK-293 treated with epidermal growth factor.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Dot	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.20 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6156
Isotype

IgG
Research areas
- Cardiovascular
- Heart
- Autophagy
- Other

Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/10000 dilution

Lane 1 : ULK1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate untreated
Lane 2 : ULK1 transfected HEK-293T cell lysate treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 112 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

The bottom panel shows total levels of ULK1 in both samples.
OI-RD Scanning - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Dot Blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

All lanes: Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/1000 dilution

Lane 1 : Human ULK1 (pS556) phospho peptide

Lane 2 : Human ULK1 non-phospho peptide

Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 112 kDa

Exposure time: 3 minutes

Blocking and diluting buffer used was 5% NFDM/TBST.
Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/5000 dilution

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HEK-293 treated with epidermal growth factor.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 112 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer used was 5% NFDM/TBST.
Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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