Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6156] to ULK1 (phospho S556)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-ULK1 (phospho S556) antibody [EPR6156]
    See all ULK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6156] to ULK1 (phospho S556)

  • Host species

    Rabbit

  • Specificity

    ab133747 only detects ULK1 phosphorylated at Serine 556.

  • Tested Applications & Species

    Application Species
    Dot
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Phospho-synthetic peptide from a region surrounding Serine 556 of Human ULK1

  • Positive control

    • WB: ULK1 transfected HEK-293T cell lysate. HEK-293 treated with epidermal growth factor.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/10000 dilution

    Lane 1 : ULK1 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate untreated
    Lane 2 : ULK1 transfected HEK-293T cell lysate treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 112 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    The bottom panel shows total levels of ULK1 in both samples.
  • OI-RD Scanning - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    OI-RD Scanning - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Dot Blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    Dot Blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

    All lanes: Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/1000 dilution

    Lane 1 : Human ULK1 (pS556) phospho peptide

    Lane 2 : Human ULK1 non-phospho peptide

     

    Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 112 kDa

    Exposure time: 3 minutes

    Blocking and diluting buffer used was 5% NFDM/TBST.

     

  • Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    Western blot - Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/5000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : HEK-293 treated with epidermal growth factor.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 112 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer used was 5% NFDM/TBST.

  • Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)
    Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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