Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15350(B)] to NDUFV2
  • Suitable for: IHC-P, WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NDUFV2 antibody [EPR15350(B)]
    See all NDUFV2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15350(B)] to NDUFV2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Ramos, Jurkat and A549 cell lysates. IHC-P: Human endometrial adenocarcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    All lanes : Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : NDUFV2 knockout HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab183717 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab183717 Anti-NDUFV2 antibody [EPR15350(B)] was shown to specifically react with NDUFV2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265619 (knockout cell lysate ab258068) was used. Wild-type and NDUFV2 knockout samples were subjected to SDS-PAGE. ab183717 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

    Immunohistochemical analysis of paraffin-embedded, Human endometrial adenocarcinoma tissue labeling NDUFV2 with ab183717 at a 1/100 dilution. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    All lanes : Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    Immunoprecipitation - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

    Western blot analysis on immunoprecipitation from 1) A549 cell lysate, or 2) TBS, labeling NDUFV2 using ab183717 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)
    Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

© 2021 Astana Biomed Group LLP. All rights reserved.