Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15350(B)] to NDUFV2
Suitable for: IHC-P, WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-NDUFV2 antibody [EPR15350(B)]
See all NDUFV2 primary antibodies
Description

Rabbit monoclonal [EPR15350(B)] to NDUFV2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Ramos, Jurkat and A549 cell lysates. IHC-P: Human endometrial adenocarcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15350(B)
Isotype

IgG
Research areas

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

All lanes : Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFV2 knockout HeLa cell lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Lanes 1-3: Merged signal (red and green). Green - ab183717 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183717 Anti-NDUFV2 antibody [EPR15350(B)] was shown to specifically react with NDUFV2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265619 (knockout cell lysate ab258068) was used. Wild-type and NDUFV2 knockout samples were subjected to SDS-PAGE. ab183717 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

Immunohistochemical analysis of paraffin-embedded, Human endometrial adenocarcinoma tissue labeling NDUFV2 with ab183717 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

All lanes : Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/20000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 27 kDa
Observed band size: 24 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

Western blot analysis on immunoprecipitation from 1) A549 cell lysate, or 2) TBS, labeling NDUFV2 using ab183717 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

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