Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22265-9] to ULK1 (phospho S757) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free
    See all ULK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22265-9] to ULK1 (phospho S757) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MEF whole cell lysate. IP: MEF whole cell lysate.

  • General notes

    Ab256537 is the carrier-free version of ab229909. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)
    Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)

    Dot blot analysis using ab229909 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 diltution.

    Lane 1: ULK1 (phospho S757) peptide.

    Lane 2: ULK1 non-phospho peptide.

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229909).

  • Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)
    Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)

    ULK1 (phospho S757) was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab229909 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab229909 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution. Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate, 10µg.

    Lane 2: ab229909 IP in MEF whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229909 in MEF whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229909).

  • Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)
    Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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