Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)
![Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)](https://www.abcam.com/ps/products/256/ab256537/Images/ab256537-2-anti-ulk1-phospho-s757-antibody-epr-22265-9-dot.jpg "Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22265-9] to ULK1 (phospho S757) - BSA and Azide free
- Suitable for: WB, Dot blot, IP
- Reacts with: Mouse
Overview
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Product name
Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free
See all ULK1 primary antibodies -
Description
Rabbit monoclonal [EPR22265-9] to ULK1 (phospho S757) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MEF whole cell lysate. IP: MEF whole cell lysate.
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General notes
Ab256537 is the carrier-free version of ab229909. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22265-9 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)
Dot blot analysis using ab229909 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 diltution.
Lane 1: ULK1 (phospho S757) peptide.
Lane 2: ULK1 non-phospho peptide.
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229909).
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![Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)](https://www.abcam.com/ps/products/256/ab256537/Images/ab256537-1-anti-ulk1-phospho-s757-antibody-epr-22265-9-immunoprecipitation-mef-mouse.jpg)
Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)ULK1 (phospho S757) was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab229909 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab229909 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution. Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.
Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate, 10µg.
Lane 2: ab229909 IP in MEF whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229909 in MEF whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229909).
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![Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)](https://www.abcam.com/ps/products/256/ab256537/Images/ab256537-3-benefits-of-recombinant-antibodies.png)
Anti-ULK1 (phospho S757) antibody [EPR22265-9] - BSA and Azide free (ab256537)
