Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)
![Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)](https://www.abcam.com/ps/products/229/ab229909/Images/ab229909-353123-anti-ulk1-phospho-s757-antibody-epr-22265-9-western-blot-mef-mouse.jpg "Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22265-9] to ULK1 (phospho S757)
- Suitable for: WB, Dot blot, IP
- Reacts with: Mouse
Overview
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Product name
Anti-ULK1 (phospho S757) antibody [EPR22265-9]
See all ULK1 primary antibodies -
Description
Rabbit monoclonal [EPR22265-9] to ULK1 (phospho S757) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MEF whole cell lysate. IP: MEF whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22265-9 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)All lanes : Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909) at 1/1000 dilution
Lane 1 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate
Lane 2 : MEF whole cell lysate (Phosphatase treated membrane)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 150 kDa why is the actual band size different from the predicted?WB membrane in lane 2 was treated with alkaline phosphatase for 1h at 37°C. This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 59 seconds.
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![Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)](https://www.abcam.com/ps/products/229/ab229909/Images/ab229909-353120-anti-ulk1-phospho-s757-antibody-epr-22265-9-western-blot-mef-mouse-1.jpg)
Western blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)All lanes : Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909) at 1/1000 dilution
Lane 1 : Untreated MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate
Lane 2 : MEF cells were cultured in amino acid and serum-free, glucose-containing starvation buffer (EBSS) for 16 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsPhosphorylation level of S757 is reduced during starvation (PMID: 28835610, 21258367). This blot was developed using a higher sensitivity ECL substrate.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 59 seconds.
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![Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)](https://www.abcam.com/ps/products/229/ab229909/Images/ab229909-2-anti-ulk1-phospho-s757-antibody-epr-22265-9-dot.jpg)
Dot Blot - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)Dot blot analysis using ab229909 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 diltution.
Lane 1: ULK1 (phospho S757) peptide.
Lane 2: ULK1 non-phospho peptide.
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)](https://www.abcam.com/ps/products/229/ab229909/Images/ab229909-1-anti-ulk1-phospho-s757-antibody-epr-22265-9-immunoprecipitation-mef-mouse.jpg)
Immunoprecipitation - Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)ULK1 (phospho S757) was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab229909 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab229909 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution. Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.
Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate, 10µg.
Lane 2: ab229909 IP in MEF whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229909 in MEF whole cell lysate.
Blocking/dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
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![Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)](https://www.abcam.com/ps/products/229/ab229909/Images/ab229909-7-benefits-of-recombinant-antibodies.png)
Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909)
