Mouse GPNMB ELISA Kit (ab270892)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 362 pg/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse GPNMB ELISA Kit
See all GPNMB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 6.7% Inter-assay Sample n Mean SD CV% Plasma 3 3.9% -
Sample type
Serum, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
362 pg/ml -
Range
0.78 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108 101% - 116% Cell culture media 81 81% - 82% EDTA Plasma 110 104% - 120% Cit plasma 103 97% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GPNMB ELISA kit (ab270892) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPNMB protein in mouse serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mouse GPNMB with 362 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
GPNMB (Glycoprotein non-metastatic gene B), also known as Osteoactivin, is a type I transmembrane glycoprotein most often associated with endosomal/lysosomal compartment and over expressed in many types of cancer. GPNMB is involved in many physiological processes, including transport of late melanosomes to keratinocytes, regulation of osteoblast and osteoclast, stimulation of dendritic cell maturation, and stimulation of adhesion of dendritic cells to endothelial cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse GPNMB Capture Antibody 1 x 600µl 10X Mouse GPNMB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse GPNMB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Could be a melanogenic enzyme. -
Tissue specificity
Not restricted to the melanocytic lineage. -
Sequence similarities
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
Developmental stage
Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. -
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Glycoprotein (transmembrane) nmb
- Glycoprotein nmb
- Glycoprotein nmb like protein
see all -
Database links
- Entrez Gene: 93695 Mouse
- SwissProt: Q99P91 Mouse
- Unigene: 302602 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse GPNMB standard curve in Sample Diluent 25BP.The GPNMB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GPNMB in mouse serum and plasma samples.The concentrations of GPNMB were measured in duplicates, interpolated from the GPNMB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GPNMB concentration was determined to be 19.4 ng/mL in neat serum, 6.5 ng/mL in plasma (citrate) and 6.6 ng/mL in plasma (EDTA).
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Sandwich ELISA - Mouse GPNMB ELISA Kit (ab270892)To learn more about the advantages of recombinant antibodies see here.
