Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse IL-11 ELISA Kit (ab215084)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 17.4 pg/ml
  • Range: 78.12 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse IL-11 ELISA Kit
    See all IL-11 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.3%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    17.4 pg/ml

  • Range

    78.12 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102 98% - 105%
    Serum 100 98% - 101%
    Cit plasma 91 86% - 97%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse IL-11 ELISA Kit (ab215084) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-11 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-11 with 17.4 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-11 is a multifunctional cytokine that is a key regulator of hematopoiesis. It stimulates hematopoietic stem cell and megakaryocyte progenitor cell proliferation. It is also involved in the response to liver damage by inducing proliferation of hepatocytes.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse IL-11 ELISA Kit (ab215084)
    Other - Mouse IL-11 ELISA Kit (ab215084)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse IL-11 standard curve in Sample Diluent NS.
    Example of mouse IL-11 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native IL-11 in mouse lung cell culture supernatant samples.
    Interpolated concentrations of native IL-11 in mouse lung cell culture supernatant samples.

    The concentrations of IL-11 were measured in duplicates and interpolated from the IL-11 standard curves. Undiluted samples are as follows: lung supernatant stimulated with TPA and IL-1a 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 2143 pg/mL in lung supernatant stimulated with TPA and IL-1a.

  • Mouse lung cells were cultured unstimulated or stimulated with 10 ng/mL TPA and 1 ng/mL IL-1a.
    Mouse lung cells were cultured unstimulated or stimulated with 10 ng/mL TPA and 1 ng/mL IL-1a.

    Conditioned media was harvested after 48 hours. IL-11 was measured in 100% unstimulated and stimulated lung supernatant. The concentrations of IL-11 were measured in duplicate, interpolated from the IL-11 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 1849 pg/mL in TPA and IL-1a stimulated lung supernatant and 809 pg/mL in unstimulated lung supernatant.

  • Sandwich ELISA - Mouse IL-11 ELISA Kit (ab215084)
    Sandwich ELISA - Mouse IL-11 ELISA Kit (ab215084)
    To learn more about the advantages of recombinant antibodies see here.

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