Anti-TNF alpha antibody (ab6671)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-TNF alpha antibody
See all TNF alpha primary antibodies -
Description
Rabbit polyclonal to TNF alpha -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6761 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.
The antibody does not recognize human TNF beta (lymphotoxin).
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Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Recombinant full length protein corresponding to Human TNF alpha.
Database link: P01375 -
Positive control
- Purchase matching WB positive control:Recombinant Human TNF alpha protein
- WB: Recombinant TNF-alpha protein.
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General notes
Endotoxin content by LAL is Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).See other anti-rabbit secondary antibodies that can be used with this antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TNF alpha antibody (ab6671)Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF alpha protein
Secondary
Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Predicted band size: 25.6 kDa
Additional bands at: 16 kDa (possible cleavage fragment)Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.
