Anti-Chk1 (phospho S345) antibody [Chk1S345-R3F9] (ab278706)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Chk1S345-R3F9] to Chk1 (phospho S345)
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Chk1 (phospho S345) antibody [Chk1S345-R3F9]
See all Chk1 primary antibodies -
Description
Rabbit monoclonal [Chk1S345-R3F9] to Chk1 (phospho S345) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human -
Immunogen
Synthetic peptide within Human Chk1 (phospho S345). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14757 -
Positive control
- Flow cyt: C6 cells (treated with pervanadate); HeLa cells (treated with Calyculin A); HeLa cells (treated with UV + TPA); NIH/3T3 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Chk1S345-R3F9 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
-
Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or untreated (red) or treated with UV plus TPA (green) using ab278706 at 1µg/mL.
-
ab278706 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278706.
-
Peptide blocking flow cytometric analysis of Hela cells secondary antibody only negative control (light blue) or untreated (red) or treated with calyculin A (green) or untreated and blocked with phospho-peptide (black) or CalA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or CalA and blocked with non-phospho peptide (purple) using ab278706 at 0.01µg/mL.
-
Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using ab278706 at 0.01µg/mL.
-