Anti-CD97 antibody [EPR4428] (ab134105)
![Anti-CD97 antibody [EPR4428] (ab134105)](https://www.abcam.com/ps/products/134/ab134105/Images/ab134105-148283-anti-cd97-antibody-epr4428-immunohistochemistry.jpg "Anti-CD97 antibody [EPR4428] (ab134105)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4428] to CD97
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD97 antibody [EPR4428]
See all CD97 primary antibodies -
Description
Rabbit monoclonal [EPR4428] to CD97 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human CD97 aa 700-800.
Database link: P48960 -
Positive control
- WB: U937 and THP-1 cell lysates IHC-P: Human colonic adenocarcinoma tissue
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4428 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4428] (ab134105)
Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue labelling CD97 with ab134105 antibody at a dilution of 1/1000.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CD97 antibody [EPR4428] (ab134105)](https://www.abcam.com/ps/products/134/ab134105/Images/ab134105-392704-anti-cd97-antibody-epr4428-westernblot-u-937-thp-1-human.jpg)
Western blot - Anti-CD97 antibody [EPR4428] (ab134105)Lane 1 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/10000 dilution
Lane 2 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/20000 dilution
Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration 5% NFDM/TBST
Exposure time:
Lane 1: 180 seconds
Lane 2: 60 secondsThe 28kDa is β-chain. This is supported by many papers, PMID: 20084281, PMID: 18941248.
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![Anti-CD97 antibody [EPR4428] (ab134105)](https://www.abcam.com/ps/products/134/ab134105/Images/ab134105-4-benefits-of-recombinant-antibodies.png)
Anti-CD97 antibody [EPR4428] (ab134105)
