Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD97 antibody [EPR4428] (ab134105)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4428] to CD97
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD97 antibody [EPR4428]
    See all CD97 primary antibodies

  • Description

    Rabbit monoclonal [EPR4428] to CD97

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human CD97 aa 700-800.
    Database link: P48960

  • Positive control

    • WB: U937 and THP-1 cell lysates IHC-P: Human colonic adenocarcinoma tissue

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4428] (ab134105)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4428] (ab134105)

    Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue labelling CD97 with ab134105 antibody at a dilution of 1/1000.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CD97 antibody [EPR4428] (ab134105)
    Western blot - Anti-CD97 antibody [EPR4428] (ab134105)
    Lane 1 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/10000 dilution
    Lane 2 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/20000 dilution

    Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 92 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Exposure time:
    Lane 1: 180 seconds
    Lane 2: 60 seconds

    The 28kDa is β-chain. This is supported by many papers, PMID: 20084281, PMID: 18941248.

  • Anti-CD97 antibody [EPR4428] (ab134105)
    Anti-CD97 antibody [EPR4428] (ab134105)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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