Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD97 antibody [EPR4427] (ab108368)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4427] to CD97
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CD97 antibody [EPR4427]
    See all CD97 primary antibodies

  • Description

    Rabbit monoclonal [EPR4427] to CD97

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of Human CD97.

  • Positive control

    • Human tonsil, Jurkat, U937, and fetal thymus lysates; Human spleen, colon adenocarcinoma and tonsil tissues. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDA-MB-231

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-CD97 antibody [EPR4427] (ab108368)
    Flow Cytometry - Anti-CD97 antibody [EPR4427] (ab108368)

    Flow Cytometry analysis of MDA-MB-231 (human mammary gland epithelial adenocarcinoma) cells labeling CD97 with purified ab108368 at 1/250 dilution (10ug/ml) (red). A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD97 antibody [EPR4427] (ab108368)
    Immunocytochemistry/ Immunofluorescence - Anti-CD97 antibody [EPR4427] (ab108368)

    ICC/IF image of ab108368 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108368 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] (ab108368)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] (ab108368)

    ab108368 at 1/250 dilution staining CD97 in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CD97 antibody [EPR4427] (ab108368)
    Western blot - Anti-CD97 antibody [EPR4427] (ab108368)
    All lanes : Anti-CD97 antibody [EPR4427] (ab108368) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : Human fetal thymus lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] (ab108368)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] (ab108368)

    ab108368 at 1/250 dilution staining CD97 in human spleen by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] (ab108368)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4427] (ab108368)

    ab108368 at 1/250 dilution staining CD97 in human colon adenocarcinoma tissue by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-CD97 antibody [EPR4427] (ab108368)
    Anti-CD97 antibody [EPR4427] (ab108368)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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