Monkey E-selectin ELISA Kit (ab119523)
Key features and details
- Sensitivity: 0.4 U/ml
- Range: 1.9 U/ml - 60 U/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey E-selectin ELISA Kit
See all CD62E kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4 Inter-assay Sample n Mean SD CV% Overall 4 -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 U/ml -
Range
1.9 U/ml - 60 U/ml -
Recovery
85 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Monkey -
Product overview
Abcam’s E-selectin monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey E-selectin concentrations in cell culture supernatant and serum.
E-selectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated E-selectin detection antibody. The microplate is then incubated at room temperature. After the removal of unbound proteins by washing TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of E-selectin captured on the plate.
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Notes
Standard is recombinant protein produced in E.coli.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 2 units E-selectin Standard lyophilized (120 U/mL upon reconstitution) 2 vials HRP Conjugated anti-E-selectin monoclonal antibody 1 x 150µl Microplate coated with monoclonal antibody to E-selectin (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD26E
- CD62 antigen-like family member E
- CD62E
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab119523.