Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]
    See all COX2 / Cyclooxygenase 2 primary antibodies

  • Description

    Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human COX2/ Cyclooxygenase 2 aa 100-200. The exact sequence is proprietary.
    Database link: P35354
    (Peptide available as ab213704)

  • Positive control

    • WB: Raw264.7 cell lysate + LPS.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Abcam recommends ab179800 for use in ICC/IF.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)

    Lane 1 : Untreated Raw 264.7 cell lysate
    Lane 2 : Raw 264.7 treated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (Purified)

    Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times

    Lane 1 and 2: 5.5 seconds
    Lane 3: 3.25 seconds

    We are unsure as to the
    identity of the extra band in HCT 116 and MCF7.
    The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, PMID: 24325753, PMID: 16997132).
    Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, PMID: 16997132)

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS

    Secondary
    Goat anti-Rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?

  • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)
    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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