Human Neutrophil Elastase ELISA Kit (ab270204)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.8 pg/ml
- Range: 47 pg/ml - 3000 pg/ml
- Sample type: Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Neutrophil Elastase ELISA Kit
See all Neutrophil Elastase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 4 4.1% Inter-assay Sample n Mean SD CV% Serum 8 3.2% -
Sample type
Serum, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.8 pg/ml -
Range
47 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 112 105% - 120% Cell culture extracts 116 115% - 117% Cell culture media 106 103% - 109% Hep Plasma 108 105% - 113% EDTA Plasma 111 103% - 118% Cit plasma 102 100% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Neutrophil Elastase ELISA Kit (ab270204) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neutrophil Elastase protein in cell culture media, cell lysate, edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neutrophil Elastase with 15.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Neutrophil Elastase, also known as bone marrow serine protease, Elastase-2, human leukocyte Elastase, and medullasin is a serine protease in the peptidase S1 family and is encoded by the ELANE gene. Neutrophil Elastase is expressed as a zymogen by neutrophils and macrophages during inflammation, where it breaks down bacterial and host membrane proteins. Neutrophil Elastase can localize to Neutrophil extracellular traps (NETs) by the protein’s affinity for DNA.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Neutrophil Elastase Capture Antibody 1 x 600µl 10X Human Neutrophil Elastase Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Neutrophil Elastase Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
Tissue specificity
Bone marrow cells. -
Involvement in disease
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
see all -
Database links
- Entrez Gene: 1991 Human
- Omim: 130130 Human
- SwissProt: P08246 Human
- Unigene: 99863 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Neutrophil Elastase standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The Neutrophil Elastase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Neutrophil Elastase in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100, plasma (EDTA) 1:50, Plasma (citrate) 1:100, and plasma (heparin)1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 225 ng/mL in serum, 113 ng/mL in plasma (EDTA), 215 ng/mL in plasma (citrate), and 291 ng/mL in plasma (heparin).
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Interpolated concentrations of native Neutrophil Elastase in human U937 cell extract based on a 2.5 µg/mL extract load.The concentrations of Neutrophil Elastase were measured in duplicate and interpolated from the Neutrophil Elastase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from eight individual healthy Neutrophil Elastase gender donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 192 ng/mL with a range of 74 – 413 ng/mL.
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Sandwich ELISA - Human Neutrophil Elastase ELISA Kit (ab270204)To learn more about the advantages of recombinant antibodies see here.
