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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14821] to TCEA1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-TCEA1 antibody [EPR14821] - BSA and Azide free
    See all TCEA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14821] to TCEA1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, SP2/0 and HUVEC cell lysate; Human bladder transitional cell carcinoma tissue; HeLa, HUVEC cells.
  • General notes

    Ab242405 is the carrier-free version of ab185947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242405 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14821
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Elongation factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

    Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

    Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)
  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

    Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)
  • Flow Cytometry - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
    Flow Cytometry - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

    Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green).  Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)
  • Immunoprecipitation - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

    Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab185947 at 1/50 dilution.  Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185947)
  • Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)
    Anti-TCEA1 antibody [EPR14821] - BSA and Azide free (ab242405)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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