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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Prohibitin antibody (ab235962)

Anti-Prohibitin antibody (ab235962)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Prohibitin
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Prohibitin antibody
    See all Prohibitin primary antibodies
  • Description

    Rabbit polyclonal to Prohibitin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow
  • Immunogen

    Recombinant full length protein corresponding to Human Prohibitin aa 1 to the C-terminus.
    Database link: P35232

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse brain lysate; Raji whole cell lysate. IP: HEK-293 whole cell lysates. IHC-P: Human kidney tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Prohibitin antibody (ab235962)
    Western blot - Anti-Prohibitin antibody (ab235962)
    Anti-Prohibitin antibody (ab235962) at 1/200 dilution + Mouse brain lysate

    Secondary
    Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-Prohibitin antibody (ab235962)
    Western blot - Anti-Prohibitin antibody (ab235962)
    Anti-Prohibitin antibody (ab235962) at 1/200 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 30 kDa

  • Immunoprecipitation - Anti-Prohibitin antibody (ab235962)
    Immunoprecipitation - Anti-Prohibitin antibody (ab235962)

    Prohibitin was immunoprecipitated from 0.5 mg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab235962 at 1/200 dilution. 

    Lane 1: Control rabbit IgG IP (1 μg) in HEK-293 whole cell lysate.

    Lane 2: ab235962 IP in HEK-293 whole cell extract.

    Lane 3: HEK-293 whole cell lysate 10 μg (Input).

  • Immunoprecipitation - Anti-Prohibitin antibody (ab235962)
    Immunoprecipitation - Anti-Prohibitin antibody (ab235962)

    Prohibitin was immunoprecipitated from 0.5 mg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab235962 at 1/200 dilution. 

    Lane 1: Control rabbit IgG IP (1 μg) in HEK-293 whole cell lysate.

    Lane 2: ab235962 IP in HEK-293 whole cell extract.

    Lane 3: HEK-293 whole cell lysate 10 μg (Input).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (ab235962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (ab235962)

    Paraffin-embedded human kidney tissue stained for Prohibitin using ab235962 at 1/20 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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