Anti-Prohibitin antibody (ab235962)
Key features and details
- Rabbit polyclonal to Prohibitin
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Prohibitin antibody
See all Prohibitin primary antibodies -
Description
Rabbit polyclonal to Prohibitin -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow -
Immunogen
Recombinant full length protein corresponding to Human Prohibitin aa 1 to the C-terminus.
Database link: P35232 -
Positive control
- WB: Mouse brain lysate; Raji whole cell lysate. IP: HEK-293 whole cell lysates. IHC-P: Human kidney tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Prohibitin antibody (ab235962) at 1/200 dilution + Mouse brain lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 30 kDa
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Anti-Prohibitin antibody (ab235962) at 1/200 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
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Prohibitin was immunoprecipitated from 0.5 mg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab235962 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HEK-293 whole cell lysate.
Lane 2: ab235962 IP in HEK-293 whole cell extract.
Lane 3: HEK-293 whole cell lysate 10 μg (Input).
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Prohibitin was immunoprecipitated from 0.5 mg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab235962 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HEK-293 whole cell lysate.
Lane 2: ab235962 IP in HEK-293 whole cell extract.
Lane 3: HEK-293 whole cell lysate 10 μg (Input).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (ab235962)
Paraffin-embedded human kidney tissue stained for Prohibitin using ab235962 at 1/20 dilution in immunohistochemical analysis.