PICK1 overexpression 293T lysate (whole cell) (ab94269)
Overview
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Product name
PICK1 overexpression 293T lysate (whole cell) -
General notes
ab94269 is a 293T cell transfected lysate in which Human PICK1 has been transiently over-expressed using a pCMV-PICK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. PTM: Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. Similarity: Contains 1 AH domain. Contains 1 PDZ (DHR) domain. Tissue specificity: Ubiquitous.
Images
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SDS-PAGE - PICK1 overexpression 293T lysate (whole cell) (ab94269)ab94269 at 15µg/lane on an SDS-PAGE gel.
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Western blot - PICK1 overexpression 293T lysate (whole cell) (ab94269)All lanes : Anti-PICK1 antibody (ab56586) at 1/500 dilution
Lane 1 :PICK1 overexpression 293T lysate (whole cell) (ab94269)
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
