Anti-CD79b antibody [CB3-1] (ab130422)
![Anti-CD79b antibody [CB3-1] (ab130422)](https://www.abcam.com/ps/products/130/ab130422/Images/ab130422-415240-anti-cd79b-antibody-cb3-1-flow-cytometry-histogram-ramos-human.jpg "Anti-CD79b antibody [CB3-1] (ab130422)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [CB3-1] to CD79b
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD79b antibody [CB3-1]
See all CD79b primary antibodies -
Description
Mouse monoclonal [CB3-1] to CD79b -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Fraction of Ig-associated molecules isolated from Ramos B cells.
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Positive control
- Flow Cyt: Human peripheral blood; Ramos cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 27th October 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CB3-1 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)
Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling CD79b with ab130422 at 1/50 (10 μg/mL) dilution (Red) compared with an Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.
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![Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)](https://www.abcam.com/ps/products/130/ab130422/Images/ab130422-415239-anti-cd79b-antibody-cb3-1-flow-cytometry-dot-plot-ramos-human.jpg)
Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)Flow cytometry staining of human PBMC for CD79b with ab130422 at 1/50 dilution (10 μg/mL) (right) or an isotype control (left). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.
