Anti-CID antibody (ab10887)
Key features and details
- Rabbit polyclonal to CID
- Suitable for: WB, ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-CID antibody -
Description
Rabbit polyclonal to CID -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Drosophila melanogasterWB Drosophila melanogaster
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Immunogen
Synthetic peptide corresponding to Drosophila melanogaster CID aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab16766) -
Positive control
- Drosophila Embryo Lysate;
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF Drosophila melanogasterWB Drosophila melanogasterApplication Abreviews Notes WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).ICC/IF 1/100.Notes WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).ICC/IF
1/100.Target
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Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. -
Sequence similarities
Belongs to the histone H3 family. -
Cellular localization
Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha. - Information by UniProt
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Database links
- Entrez Gene: 36495 Drosophila melanogaster
- SwissProt: Q9V6Q2 Drosophila melanogaster
- Unigene: 5691 Drosophila melanogaster
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Alternative names
- CENP A antibody
- CENP-A homolog antibody
- CENPA / CID antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (ab10887)This image is courtesy of Elaine DunleavyDrosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
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Western blot - Anti-CID antibody (ab10887)Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
Protocols
Datasheets and documents
References (11)
ab10887 has been referenced in 11 publications.
- Huang A et al. Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading. Nucleic Acids Res 47:10754-10770 (2019). PubMed: 31535131
- Pavlova GA et al. RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis. PLoS Genet 15:e1008371 (2019). PubMed: 31527906
- Strunov A et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol 16:68 (2018). PubMed: 29907103
- Moshkin YM et al. Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A. PLoS Genet 9:e1003719 (2013). PubMed: 24086141
- Yan R et al. SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster. J Cell Biol 188:335-49 (2010). ICC/IF ; Drosophila melanogaster . PubMed: 20142422
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (ab10887) This image is courtesy of Elaine DunleavyDrosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
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Western blot - Anti-CID antibody (ab10887)Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)