Anti-EBF1 antibody [EPR4183] (ab108369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4183] to EBF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EBF1 antibody [EPR4183]
See all EBF1 primary antibodies -
Description
Rabbit monoclonal [EPR4183] to EBF1 -
Host species
Rabbit -
Specificity
ab108369 also recognises EBF3 protein.
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Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Purchase matching WB positive control:Recombinant Human EBF1 protein
- WB: Raji, Daudi and 3T3-LI cell lysates. Mouse and rat spleen and lymph node tissue lysates.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4183 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse lymph node lysate
Lane 5 : Rat spleen lysate
Lane 6 : Rat lymph node lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaWe are unsure how to define the extra bands.
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Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (unpurifed) + Raji cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 20 µg
Predicted band size: 64 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-EBF1 antibody [EPR4183] (ab108369) at 1/1000 dilution (unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : 3T3-LI cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
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