Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD80 antibody [EPR1157(2)] (ab134120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1157(2)] to CD80
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD80 antibody [EPR1157(2)]
    See all CD80 primary antibodies

  • Description

    Rabbit monoclonal [EPR1157(2)] to CD80

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD80 aa 250 to the C-terminus.

  • Positive control

    • WB: Raji and Ramos cell lysates. IHC-P: human tonsil Tissue Flow Cyt: Raji cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD80 with purified ab134120 at 1:190 dilution (10.33 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab134120 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Trs/EDTA buffer, pH9 (ab93684). ImmunoHistoProbe one step HRP Polymer was used. Counter stained with hematoxylin.

    The image shows cytoplasmic and membrane staining.

  • Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution

    Lane 1 : Wild-type Raji cell lysate
    Lane 2 : CD80 knockout Raji cell lysate
    Lane 3 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab134120 observed at 55 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

    ab134120 was shown to react with CD80 in wild-type Raji cells in western blot with loss of signal observed in CD80 knockout sample. Wild-type and CD80 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134120 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)
    All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Anti-CD80 antibody [EPR1157(2)] (ab134120)
    Anti-CD80 antibody [EPR1157(2)] (ab134120)

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