Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP498Y] to CD22 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD22 antibody [EP498Y] - BSA and Azide free
    See all CD22 primary antibodies

  • Description

    Rabbit monoclonal [EP498Y] to CD22 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises CD22. 

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247276 is the carrier-free version of ab33859. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247276 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    All lanes : Anti-CD22 antibody [EP498Y] (ab33859) at 1/1000 dilution (purified)

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : RAW 264.7 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 95 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab33859, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    Anti-CD22 antibody [EP498Y] (ab33859) at 1/5000 dilution (purified) + Raji whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 95 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    This data was developed using ab33859, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    Western blot - Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    Anti-CD22 antibody [EP498Y] (ab33859) at 1/1000 dilution (unpurified) + Raji cell lysate

    Predicted band size: 95 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    This data was developed using ab33859, the same antibody clone in a different buffer formulation.

     

  • Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)
    Anti-CD22 antibody [EP498Y] - BSA and Azide free (ab247276)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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