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Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)

Key features and details

  • Mouse monoclonal [5G6B4] to ErbB4 / HER4 - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal
    See all ErbB4 / HER4 primary antibodies

  • Description

    Mouse monoclonal [5G6B4] to ErbB4 / HER4 - C-terminal

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human ErbB4/ HER4 aa 1150 to the C-terminus (C terminal). Expressed in E. coli.
    Database link: Q15303

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human ErbB 4 (amino acids 1159-1308) recombinant protein. Lysate from HEK293 cells transfected with ErbB 4 (amino acids 1159-1308). Human cervical cancer tissue. HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)
    Western blot - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)
    Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959) at 1/500 dilution + human ErbB 4 (amino acids 1159-1308) recombinant protein.

    Predicted band size: 147 kDa



    (Expected MW is 43.3 kDa)

  • Western blot - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)
    Western blot - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)
    All lanes : Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959) at 1/500 dilution

    Lane 1 : HEK293 cell lysate.
    Lane 2 : Lysate from HEK293 cells transfected with ErbB 4 (amino acids 1159-1308)-hIgGFc.

    Predicted band size: 147 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)

    Immunohistochemical analysis of paraffin-embedded, DAB-stained human cervical cancer tissue, labeling ErbB4 / HER4 using ab204959 at a 1/200 dilution

  • Flow Cytometry - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)
    Flow Cytometry - Anti-ErbB4 / HER4 antibody [5G6B4] - C-terminal (ab204959)

    Flow cytometry analysis of HeLa cells labeling ErbB4 / HER4 (green) using ab204959 at a 1/200 dilution, and negative control cells (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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