Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)
Key features and details
- Rabbit polyclonal to ErbB4 / HER4 (phospho Y1284)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1284) antibody
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit polyclonal to ErbB4 / HER4 (phospho Y1284) -
Host species
Rabbit -
Specificity
Detects endogenous levels of ErbB4 / HER4 only when phosphorylated at tyrosine 1284.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human ErbB4/ HER4. Synthetic phosphopeptide derived from human ErbB4/ HER4 around the phosphorylation site of tyrosine 1284 (P-E-YP-L-S)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059) This image is courtesy of an Abreview submitted by Simon Pascual
ab61059 staining ErbB4 / HER4 (phospho Y1284) in rat heart tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with paraformaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% BSA + normal goat serum for 4 hours at 21°C. Samples were incubated with primary antibody (1/30 in 5% BSA + 0.05% Tween20 in TBS) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/200) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)1/100-1/500 ab61059 staining phosphorilated ErbB4 / HER4 on HeLa cells treated with 200nM EGF for 5 minutes, in the absence (left) or presence (right) of the immunizing phosphopeptide.
