Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E7] to Caspase-8 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-8 antibody [E7] - BSA and Azide free
    See all Caspase-8 primary antibodies

  • Description

    Rabbit monoclonal [E7] to Caspase-8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type/ Wild type treated with Staurosporin HAP1, Jurkat, IM-9, SH-SY5Y, and HeLa (ab150035) cell lysates.

  • General notes

    ab232046 is the carrier-free version of ab32397 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232046 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)
    Western blot - Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)
    All lanes : Anti-Caspase-8 antibody [E7] (ab32397) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CASP8 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab32397).

      Lanes 1- 4: Merged signal (red and green). Green - ab32397 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32397 was shown to react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type HeLa and CASP8 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)
    Western blot - Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)
    All lanes : Anti-Caspase-8 antibody [E7] (ab32397) at 1/500 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Wild-type HAP1 whole cell lysate treated with Staurosporin
    Lane 3 : Caspase-8 knockout HAP1 whole cell lysate
    Lane 4 : Caspase-8 knockout HAP1 whole cell lysate treated with Staurosporin

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 55 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32397 observed at 55, 43/41 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32397 was shown to specifically react with HAP1 + Staurosproin when HAP1 + Staurosproin knockout samples were used. Wild-type and HAP1 + Staurosproin knockout samples were subjected to SDS-PAGE. ab32397 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32397).

     

  • Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)
    Anti-Caspase-8 antibody [E7] - BSA and Azide free (ab232046)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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