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Anti-Caspase-8 antibody [ABM14C1] (ab220171)

Key features and details

  • Mouse monoclonal [ABM14C1] to Caspase-8
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Caspase-8 antibody [ABM14C1]
    See all Caspase-8 primary antibodies

  • Description

    Mouse monoclonal [ABM14C1] to Caspase-8

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Caspase-8 aa 150-400.
    Database link: Q14790

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Molt-4, Kato III and HepG2 cell lysate. IHC-P: Human esophagus and tonsil tissue. Flow Cyt: HeLa and Jurkat cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody [ABM14C1] (ab220171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody [ABM14C1] (ab220171)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human esophagus tissue sections labeling Caspase-8 with ab220171 at 5 µg/mL.

  • Western blot - Anti-Caspase-8 antibody [ABM14C1] (ab220171)
    Western blot - Anti-Caspase-8 antibody [ABM14C1] (ab220171)
    All lanes : Anti-Caspase-8 antibody [ABM14C1] (ab220171) at 2 µg/ml

    Lane 1 : Molt-4 cell lysate
    Lane 2 : Kato III cell lysate
    Lane 3 : HepG2 cell lysate

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody [ABM14C1] (ab220171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody [ABM14C1] (ab220171)

    Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue sections labeling Caspase-8 with ab220171 at 5 µg/mL.

  • Flow Cytometry - Anti-Caspase-8 antibody [ABM14C1] (ab220171)
    Flow Cytometry - Anti-Caspase-8 antibody [ABM14C1] (ab220171)

    Flow Cytometry analysis of HeLa cells labeling Caspase-8 with ab220171 at 0.5 µg/106 cells.

    ab220171: red

    Isotype control: green

    A goat anti-mouse PE conjugate was used as the secondary antibody.

  • Flow Cytometry - Anti-Caspase-8 antibody [ABM14C1] (ab220171)
    Flow Cytometry - Anti-Caspase-8 antibody [ABM14C1] (ab220171)

    Flow Cytometry analysis of Jurkat cells lableing Caspase-8 with ab220171 at 0.5 µg/106 cells.

    ab220171: red

    Isotype control: green

    A goat anti-mouse PE conjugate was used as the secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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