Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22073] to Beta Arrestin 2
Suitable for: WB, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Beta Arrestin 2 antibody [EPR22073]
See all Beta Arrestin 2 primary antibodies
Description

Rabbit monoclonal [EPR22073] to Beta Arrestin 2
Host species

Rabbit
Specificity

The immunogen used for this product shares 86% homology with Beta Arrestin 1. Cross-reactivity with this protein has not been confirmed experimentally.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T, HepG2, Jurkat and HeLa whole cell lysates; Human lung and brain lysates; Mouse and rat brain lysates. Flow Cyt: HeLa cells. IP: HEK-293T whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22073
Isotype

IgG
Research areas

Western blot - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

All lanes : Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 46 kDa
Observed band size: 46-55 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The multiple bands in the brain tissue lysate are different isoform of β-Arrestin 2. All the immuno- active bands can be totally blocked by pre-incubation of antigen peptide.
Western blot - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

All lanes : Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Human lung lysate
Lane 4 : Human brain lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat brain lysate
Lane 7 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

Predicted band size: 46 kDa
Observed band size: 46-55 kDa why is the actual band size different from the predicted?

Exposure time : Lane 1 - Lane 6: 10 seconds, Lane 7: 48 seconds, Lane 8: 70 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The multiple bands in tissue lysates are different isoforms of β-Arrestin 2 based on UniProt annotation. The molecular profile/weight observed is consistent with what has been described in the literature (PMID:16820410, PMID:27759077, PMID:19955404).
Immunoprecipitation - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

Beta Arrestin 2 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab206972 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206972 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: HEK-293T whole cell lysate 10 µg (Input).

Lane 2: ab206972 IP in HEK-293T whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206972 in HEK-293T whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.
Flow Cytometry - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Beta Arrestin 2 with ab206972 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

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