Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22073] to Beta Arrestin 2
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Beta Arrestin 2 antibody [EPR22073]
    See all Beta Arrestin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22073] to Beta Arrestin 2

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 86% homology with Beta Arrestin 1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HepG2, Jurkat and HeLa whole cell lysates; Human lung and brain lysates; Mouse and rat brain lysates. Flow Cyt: HeLa cells. IP: HEK-293T whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22073

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    Western blot - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    All lanes : Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46-55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The multiple bands in the brain tissue lysate are different isoform of β-Arrestin 2. All the immuno- active bands can be totally blocked by pre-incubation of antigen peptide.

  • Western blot - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    Western blot - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    All lanes : Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : Human lung lysate
    Lane 4 : Human brain lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Rat brain lysate
    Lane 7 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46-55 kDa why is the actual band size different from the predicted?



    Exposure time : Lane 1 - Lane 6: 10 seconds, Lane 7: 48 seconds, Lane 8: 70 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The multiple bands in tissue lysates are different isoforms of β-Arrestin 2 based on UniProt annotation. The molecular profile/weight observed is consistent with what has been described in the literature (PMID:16820410, PMID:27759077, PMID:19955404).

  • Immunoprecipitation - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    Immunoprecipitation - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

    Beta Arrestin 2 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab206972 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab206972 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HEK-293T whole cell lysate 10 µg (Input). 

    Lane 2: ab206972 IP in HEK-293T whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206972 in HEK-293T whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Flow Cytometry - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    Flow Cytometry - Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Beta Arrestin 2 with ab206972 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)
    Anti-Beta Arrestin 2 antibody [EPR22073] (ab206972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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