Anti-VAV1 antibody (ab97574)
Key features and details
- Rabbit polyclonal to VAV1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VAV1 antibody
See all VAV1 primary antibodies -
Description
Rabbit polyclonal to VAV1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 83 and 538 of Human VAV1 (NP_005419)
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Positive control
- WB: Jurkat, NCI-H929 and BCL-1 whole cell lysate. IHC-P: Human serous ovarian cancer.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-VAV1 antibody (ab97574) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
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Paraffin-embedded human serous ovarian cancer tissue stained VAV1 using ab97574 at 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min
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Anti-VAV1 antibody (ab97574) at 1/1000 dilution + BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
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All lanes : Anti-VAV1 antibody (ab97574) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE