Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174) - BSA and Azide free
  • Suitable for: WB, Flow Cyt, Dot blot, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free
    See all VAV1 primary antibodies

  • Description

    Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects VAV1 phosphorylated at Tyrosine 174.

  • Tested applications

    Suitable for: WB, Flow Cyt, Dot blot, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Jurkat cells treated with Pervanadate (1 mM, 30 minutes).

  • General notes

    ab238424 is the carrier-free version of ab76225 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238424 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)
    Flow Cytometry - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76225).

  • Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)
    Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)

    Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76225).

  • Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)
    Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)

    Immunocytochemistry/Immunofluorescence analysis of untreated Jurkat cells and Pervanadate (1 mM, 30 minutes) treated Jurkat cells labeling VAV1 (phospho Y174) with purified ab76225 at 1/500 dilution.

    Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1:1000 (1 µg/ml). An Alexa-Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

    Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120).

    Negative Control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077).

    This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, tissue culture supernatant, BSA, glycerol and sodium azide (ab76225).

  • Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)
    Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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