Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] - BSA and Azide free (ab270617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R29-52] to LRRK2 (phospho T1357) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] - BSA and Azide free
    See all LRRK2 primary antibodies

  • Description

    Rabbit monoclonal [MJF-R29-52] to LRRK2 (phospho T1357) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Immunoprecipitates of overexpressed FLAG-LRRK2 G2019S in HEK293 cells, subjected to an in vitro kinase reaction prior to SDS-PAGE analysis.

  • General notes

    ab270617 is the carrier-free version of ab270606. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab270617 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed with support from The Michael J. Fox Foundation.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] - BSA and Azide free (ab270617)
    Western blot - Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] - BSA and Azide free (ab270617)
    All lanes : Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] (ab270606) at 1/1000 dilution

    Lane 1 : Immunoprecipitates of overexpressed FLAG-LRRK2 G2019S in HEK293 cells, subjected to an in vitro kinase reaction prior to SDS-PAGE analysis
    Lane 2 : Immunoprecipitates of overexpressed FLAG-LRRK2 D2017A in HEK293 cells, subjected to an in vitro kinase reaction prior to SDS-PAGE analysis

    Lysates/proteins at 10 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 286 kDa
    Observed band size: 288 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab270606, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The G2019S leucine-rich repeat kinase 2 (LRRK2) mutation is the most common cause of genetic Parkinson's disease (PD). Transfection of G2019S mutant can lead to the phosphorylation of LRRK2 at Thr1357.

    The immunoprecipitates were kindly provided by Dr. Dario Alessi.

    Exposure time: 3 minutes.

  • Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] - BSA and Azide free (ab270617)
    Anti-LRRK2 (phospho T1357) antibody [MJF-R29-52] - BSA and Azide free (ab270617)

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