Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free (ab205335)
![Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free (ab205335)](https://www.abcam.com/ps/products/205/ab205335/Images/ab205335-410894-anti-lrrk2-antibody-western-blot-hap1-human.jpg "Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free (ab205335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJFF4 (c81-8)] to LRRK2 - Low endotoxin, Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free
See all LRRK2 primary antibodies -
Description
Rabbit monoclonal [MJFF4 (c81-8)] to LRRK2 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human LRRK2 aa 950 to the C-terminus.
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General notes
ab205335 is the Low endotoxin, azide-free version of ab133476 This format is designed for in vitro and in vivo studies, including neutralization, blocking or activation/proliferation.
Our Low endotoxin, azide-free formats [add hyperlink] have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJFF4 (c81-8) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free (ab205335)
This data was developed using the same antibody clone in a different buffer formulation (ab133476).
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LRRK2 knockout HAP1 cell lysate (20 µg)
Lane 3: MEF cell lysate (20 µg)
Lane 4: LRRK2 knockout MEF cell lysate (20 µg)
Lane 5: A549 cell lysate (20ug)
Lane 6: LRRK2 knockout A549 cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green – ab133476 observed at 238 kDa. Red - loading control, ab130007, observed at 124 kDa.
ab133476 was shown to specifically react with LRRK2 in wild type A549, MEF and HAP1 cells along with additional cross-reactive bands. No band was observed when LRRK2 knockout samples were used. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. ab133476 and ab130007 (loading control to Vinculin) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Wild-type and LRRK2 knockout MEF and A549 cells were provide as a generous gift from Professor Dario Alessi, MRC Protein Phosphorylation and Ubiquitination Unit (University of Dundee).
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![Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free (ab205335)](https://www.abcam.com/ps/products/205/ab205335/Images/ab205335-3-benefits-of-recombinant-antibodies.png)
Anti-LRRK2 antibody [MJFF4 (c81-8)] - Low endotoxin, Azide free (ab205335)
